Publications

Journal Article

Torben Mentrup, Kosta Theodorou, Florencia Cabrera-Cabrera, Andreas O. Helbig, Kathrin Happ, Marion Gijbels, Ann-Christine Gradtke, Björn Rabe, Akio Fukumori, Harald Steiner, Andreas Tholey, Regina Fluhrer, Marjo Donners, Bernd Schröder (2019)
Atherogenic LOX-1 signaling is controlled by SPPL2-mediated intramembrane proteolysis
J Exp Med. 2019 Apr 1;216(4):807-830
Alkmini A Papadopoulou, Stephan A Müller, Torben Mentrup, Merav D Shmueli, Johannes Niemeyer, Martina Haug-Kröper, Julia von Blume, Artur Mayerhofer, Regina Feederle, Bernd Schröder, Stefan F Lichtenthaler, Regina Fluhrer (2019)
Signal Peptide Peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins
EMBO Rep. 2019 May;20(5). pii: e48133
Johannes Niemeyer, Torben Mentrup, Ronny Heidasch, Stephan A Müller, Uddipta Biswas, Rieke Meyer, Alkmini A Papadopoulou, Verena Dederer, Martina Haug-Kröper, Vivian Adamski, Renate Lüllmann-Rauch, Martin Bergmann, Artur Mayerhofer, Paul Saftig, Gunther Wennemuth, Rolf Jessberger, Regina Fluhrer, Stefan F Lichtenthaler, Marius K Lemberg, Bernd Schröder (2019)
The intramembrane protease SPPL2c promotes male germ cell development by cleaving phospholamban
EMBO Rep. 2019 Feb 7
Jules F, Sauvageau E, Dumaresq-Doiron K, Mazzaferri J, Haug-Kröper M, Fluhrer R, Costantino S, Lefrancois S (2017)
CLN5 is cleaved by members of the SPP/SPPL family to produce a mature soluble protein
Exp Cell Res. 2017 Aug 1;357(1):40-50
Mentrup T, Fluhrer R, Schröder B (2017)
Latest emerging functions of SPP/SPPL intramembrane proteases
Eur J Cell Biol. 2017 Aug;96(5):372-382
Mentrup T, Loock AC, Fluhrer R, Schröder B (2017)
Signal peptide peptidase and SPP-like proteases - Possible therapeutic targets?
Biochim Biophys Acta. 2017 Nov;1864(11 Pt B):2169-2182.
Susann Hüttl, Felix Helfrich, Torben Mentrup, Sebastian Held, Harald Steiner, Paul Saftig, Regina Fluhrer, Bernd Schröder (2016)
Substrate determinants of signal peptide peptidase-like 2a (SPPL2a)-mediated intramembrane proteolysis of the invariant chain CD74
Biochem J. 2016 May 15;473(10):1405-22.
Mentrup T, Häsler R, Fluhrer R, Saftig P, Schröder B (2015)
A Cell-Based Assay Reveals Nuclear Translocation of Intracellular Domains Released by SPPL Proteases
Traffic. 2015 Aug;16(8):871-92. doi: 10.1111/tra.12287. Epub 2015 May 6
Frits Kamp, Edith Winkler, Johannes Trambauer, Amelie Ebke, Regina Fluhrer, Harald Steiner (2015)
Intramembrane proteolysis of β-amyloid precursor protein by γ-secretase is an unusually slow process
Biophys J. 2015 Mar 10;108(5):1229-37.
Fleck D, Voss M, Brankatschk B, Giudici C, Hampel H, Edbauer D, Steiner H, Kremmer E, Haug-Kröper M, Rossner MJ, Fluhrer R, Willem M, Haass C (2015)
Proteolytic processing of neuregulin 1 type III by three intramembrane cleaving proteases
J Biol Chem. 2016 Jan 1;291(1):318-33.
Kuhn PH, Voss M, Haug-Kröper M, Schröder B, Schepers U, Bräse S, Haass C, Lichtenthaler SF, Fluhrer R (2015)
Secretome analysis identifies novel signal Peptide peptidase-like 3 (Sppl3) substrates and reveals a role of Sppl3 in multiple Golgi glycosylation pathways
Mol Cell Proteomics. 2015 Jun;14(6):1584-98. doi: 10.1074/mcp.M115.048298. Epub 2015 Mar 31.
Fluhrer R (2014)
Intramembrane cleaving proteases (I-CLiPs) as guardians of shuttling proteins
Cell Cycle. 2014 Feb 4;13(5). [Epub ahead of print]
Voss M, Künzel U, Higel F, Kuhn PH, Colombo A, Haug-Kröper M, Klier B, Grammer G, Seidl A, Schröder B, Obst R, Steiner H, Lichtenthaler SF, Haass C, Fluhrer R (2014)
Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation
EMBO J. 2014 Dec 17;33(24):2890-905. doi: 10.15252/embj.201488375. Epub 2014 Oct 29.
Schneppenheim J, Hüttl S, Kruchen A, Fluhrer R, Müller I, Saftig P, Schneppenheim R, Martin CL, Schröder B (2014)
Signal-peptide-peptidase-like 2a is required for CD74 intramembrane proteolysis in human B cells
Biochem Biophys Res Commun. 2014 Aug 15;451(1):48-53. doi: 10.1016/j.bbrc.2014.07.051. Epub 2014 Jul 15.
Schneppeneim J, Hüttl S, Mentrup T, Lüllmann-Rauch R, Rothaug M, Engelke M, Dittmann K, Dressel R, Araki M, Araki K, Wienands J, Fluhrer R, Saftig P, Schröder B (2014)
The intramembrane proteases Signal-peptide-peptidase-like 2a and b (SPPL2a/b) have distinct functions in vivo
Mol Cell Biol. 2014 Feb 3. [Epub ahead of print]
Voss M, Schröder B, Fluhrer R (2013)
Mechanism, specificity, and physiology of signal peptide peptidase (SPP) and SPP-like proteases.
Biochim Biophys Acta. 2013 Dec;1828(12):2828-39. doi: 10.1016/j.bbamem.2013.03.033.
Poggi M, Kara I, Brunel JM, Landrier JF, Govers R, Bonardo B, Fluhrer R, Haass C, Alessi MC, Peiretti F (2013)
Palmitoylation of TNF alpha is involved in the regulation of TNF receptor 1 signalling
Biochim Biophys Acta. 2013 Mar;1833(3):602-12. doi: 10.1016/j.bbamcr.2012.11.009. Epub 2012 Nov 16.
Bronckers AL, Gueneli N, Lüllmann-Rauch R, Schneppeneim J, Moraru AP, Himmerkus N, Bervoets TJ, Fluhrer R, Everts V, Saftig P, Schröder B (2013)
The intramembrane protease SPPL2A is critical for tooth enamel formation
J Bone Miner Res. 2013 Jul;28(7):1622-30. doi: 10.1002/jbmr.1895
Schneppeneim J, Dressel R, Hüttl S, Lüllmann-Rauch R, Engelke M, Dittmann K, Wienands J, Eskelinen EL, Hermans-Borgmeyer I, Fluhrer R, Saftig P, Schröder B (2013)
The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain
J Exp Med. 2013 Jan 14;210(1):41-58. doi: 10.1084/jem.20121069. Epub 2012 Dec 24.
Zahn C, Kaup M, Fluhrer R, Fuchs H (2013)
The transferrin receptor-1 membrane stub undergoes intramembrane proteolysis by signal peptide peptidase-like 2b
FEBS J. 2013 Apr;280(7):1653-63. doi: 10.1111/febs.12176. Epub 2013 Mar 1.
Voss M, Kuhn PH, Künzel U, Klier B, Haug-Kröper M, Kremmer E, Lichtenthaler SF, Steiner H, Schröder B, Fluhrer R (2012)
Foamy virus envelope protein is a substrate for signal peptide peptidase-like 3 (SPPL3)
J Biol Chem. 2012 Dec 21;287(52):43401-9. doi: 10.1074/jbc.M112.371369. Epub 2012 Nov 6.
Fluhrer R, Kamp F, Nuscher B, Steiner H, Beyer K, Haass C (2011)
The nicastrin ectodomain adopts a highly thermostable structure
Biol Chem. 2011 Aug 18. [Epub ahead of print]
Fluhrer R, Martin L, Haug-Kroeper M, Nuscher B, Haass C (2011)
The α-helical content of the transmembrane domain of the British dementia protein-2 (Bri2) determines its processing by Signal Peptide Peptidase-Like 2b (SPPL2b)
J Biol Chem. 2012 Feb 10;287(7):5156-63. Epub 2011 Dec 22
Fluhrer R, Steiner H, Haass C (2010)
Three-amino acid spacing of presenilin endoproteolysis suggests a general stepwise cleavage of γ-secretase-mediated intramembrane proteolysis
J. Neurosci. 2010 Apr 20. [Epub ahead of print]
Fluhrer R, Steiner H, Haass C (2010)
Three-amino acid spacing of presenilin endoproteolysis suggests a general stepwise cleavage of γ-secretase-mediated intramembrane proteolysis
J Neurosci. 2010 Jun 9;30(23):7853-62.
Fluhrer R, Martin L, Grammer G, Haug-Kroper M, Klier B, Winkler E, Kremmer E, Condron MM, Teplow DB, Steiner H, Haass C (2008)
Intramembrane proteolysis of GXGD-type aspartyl proteases is slowed by a familial Alzheimer disease-like mutation
J Biol Chem 283(44): 30121-8
Martin L, Fluhrer R, Reiss K, Kremmer E, Saftig P, Haass C (2008)
Regulated intramembrane proteolysis of Bri2 (Itm2b) by ADAM10 and SPPL2a/SPPL2b
J Biol Chem 283(3): 1644-52
Martin L, Fluhrer R, Haass C (2008)
Substrate requirements for SPPL2b dependent regulated intramembrane proteolysis
J Biol Chem
Prager K, Wang-Eckhardt L, Fluhrer R, Killick R, Barth E, Hampel H, Haass C, Walter J (2007)
A structural switch of presenilin 1 by glycogen synthase kinase 3beta-mediated phosphorylation regulates the interaction with beta-catenin and its nuclear signaling
J Biol Chem 282(19): 14083-93
Fluhrer R, Grammer G, Israel L, Condron MM, Haffner C, Friedmann E, Bohland C, Imhof A, Martoglio B, Teplow DB, Haass C (2006)
A gamma-secretase-like intramembrane cleavage of TNFalpha by the GxGD aspartyl protease SPPL2b
Nat Cell Biol 8(8): 894-6
Krawitz P, Haffner C, Fluhrer R, Steiner H, Schmid B, Haass C (2005)
Differential localization and identification of a critical aspartate suggest non-redundant proteolytic functions of the presenilin homologues SPPL2b and SPPL3
J Biol Chem 280(47): 39515-23
Fluhrer R, Friedlein A, Haass C, Walter J (2004)
Phosphorylation of presenilin 1 at the caspase recognition site regulates its proteolytic processing and the progression of apoptosis
J Biol Chem 279(3): 1585-93
Fluhrer R, Multhaup G, Schlicksupp A, Okochi M, Takeda M, Lammich S, Willem M, Westmeyer G, Bode W, Walter J, Haass C (2003)
Identification of a beta-secretase activity, which truncates amyloid beta-peptide after its presenilin-dependent generation
J Biol Chem 278(8): 5531-8
Fluhrer R, Capell A, Westmeyer G, Willem M, Hartung B, Condron MM, Teplow DB, Haass C, Walter J (2002)
A non-amyloidogenic function of BACE-2 in the secretory pathway
J Neurochem 81(5): 1011-20
Capell A, Meyn L, Fluhrer R, Teplow DB, Walter J, Haass C (2002)
Apical sorting of beta-secretase limits amyloid beta-peptide production
J Biol Chem 277(7): 5637-43
Walter J, Fluhrer R, Hartung B, Willem M, Kaether C, Capell A, Lammich S, Multhaup G, Haass C (2001)
Phosphorylation regulates intracellular trafficking of beta-secretase
J Biol Chem 276(18): 14634-41