Ludwig-Maximilians-Universität, Chair of Metabolic Biochemistry

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An in vivo assay for the identification of target proteases which cleave membrane-associated substrates

FEBS Lett 463(3): 245-9

Authors/Editors: Steiner H
Pesold B
Haass C
Publication Date: 1999
Type of Publication: Journal Article

Proteases not only play a fundamental role in numerous physiological processes, but are also involved in several human diseases including Alzheimer's disease (AD). A key protease implicated in AD is the so far unidentified γ-secretase, which cleaves the membrane-bound β-amyloid precursor protein (βAPP) at the C-terminus of its amyloid domain within the membrane to release the neurotoxic amyloid β-peptide. In order to allow the isolation of proteases, which specifically cleave membrane-bound substrates within or in the vicinity of a transmembrane domain, we developed a reporter gene assay in Saccharomyces cerevisiae. This assay may allow the identification of genes encoding target proteases that specifically cleave membrane bound substrates by transforming expression libraries.

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