Ludwig-Maximilians-Universität, Chair of Metabolic Biochemistry

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'Semifree-floating' treatment: a simple and fast method to process consecutive sections for immunohistochemistry and neuronal tracing

J Neurosci Methods 72(1): 57-63

Authors/Editors: Herzog A
Brosamle C
Publication Date: 1997
Type of Publication: Journal Article
A method is described which allows for histochemical processing of thick (50-200 microm) and consecutive sections of neural tissue, a prerequisite for many neuroanatomical studies. Two examples are given: (A) biotin-dextran-amine (BDA) tracing of neuronal connections in 50-100 microm thick vibratome sections of the adult rat brain and (B) immunohistochemical analysis of tyrosine hydroxylase-positive bulbospinal fibers in 50 microm thick cryosections of spinal cord.

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